講演詳細

動物・昆虫部門[Forest Insects and Animals]

日付	ポスター発表
講演番号	P-411
発表題目	Screening and insecticidal mechanism study of Bacillus thuringiensis Cry toxins against pinewood nematode Screening and insecticidal mechanism study of Bacillus thuringiensis Cry toxins against pinewood nematode
要旨本文	Bursaphelenchus xylophilus causes pine wilt disease, which results in severe ecological and economic losses. Effective control methods for the pinewood nematode are urgently required. Bacillus thuringiensis, a widely used microbial insecticide, produces toxins that are toxic to several species of parasitic nematodes; but the nematocidal activity to pinewood nematodes is unknown. Therefore, the effects of nine nematocidal toxins on pinewood nematodes were studied. First, the results showed six toxins with high toxicity, including App6Aa2, Cry13Aa1, Cry12Aa1, Cry5Ba3, Xpp55Aa1, and Cyt8Aa1. Then, the results of enzymatic activation indicated insufficient processing or non-specific enzymatic digestion of nematocidal toxins by the gut proteases of B. xylophilus. Besides, App6Aa1, Xpp55Aa1, and Cyt8Aa1 toxins can bind more proteins in BBMV to other toxins. The results of this study provide the direction for the use of nematocidal toxins in the biological control of B. xylophilus.
著者氏名	○GUO, Yajie1 ・ WU, Songqing2 ・ LIAN, Chunlan1
著者所属	1The University of Tokyo ・ 2Fujian Agriculture and Forestry University
キーワード	Pinewood nematode, Bacillus thuringiensis, Cry toxins, Insecticidal activity
Key word	Pinewood nematode, Bacillus thuringiensis, Cry toxins, Insecticidal activity