第134回日本森林学会大会 発表検索
講演詳細
微生物部門[Forest Microbes]
日付 | ポスター発表 |
---|---|
会場名 | (学生ポスター賞の審査対象) |
講演番号 | P-431 |
発表題目 | Screening candidate genes and exploring their functions related to ectomycorrhizal formation in Populus tomentosa Screening candidate genes and exploring their functions related to ectomycorrhizal formation in Populus tomentosa |
要旨本文 | Ectomycorrhizal (ECM) fungi are preferentially form symbionts with many woody plants. In our previous study, 173 differentially expressed genes (DEGs) of Populus tomentosa involved in the initial stage of ECM formation by Cenococcum geophilum were identified by RNA-sequencing. Of these DEGs, the expression of four genes (PtPE15, PtPE17, PtGPS1104, and PtGST2) in wild-type and ECM seedlings were assessed by real-time quantitative PCR in this research. To reveal these four genes how to regulate ECM formation, overexpression and RNAi methods were used to obtain mutant seedlings of P. tomentosa. The regenerated transgenic seedlings are in progress. The inoculation experiment and the exploration of functions of the four genes will be conducted in the near future. The result of this research may provide some insight in the ECM formation in poplar. |
著者氏名 | ○Ying Liu1 ・ Chaofeng Li2 ・ Yajie Guo1 ・ Yuanxun Tao1 ・ Norihisa Matsushita1 ・ Chunlan Lian2 |
著者所属 | 1Graduate School of Agricultural and Life Sciences, The University of Tokyo ・ 2Asian Research Center for Bioresource and Environmental Sciences, The University of Tokyo |
キーワード | ectomycorrhiza, populus tomentosa, gene functions |
Key word | ectomycorrhiza, populus tomentosa, gene functions |