| 日付 |
2024年3月8-10日 |
| 開始時刻 |
ポスター発表 |
| 会場名 |
542 |
| 講演番号 |
GP-02 |
| 発表題目 |
Development of on-site quick diagnosis system for detecting Phytoplasma related diseases in South Korea
|
| 所属 |
National Institute of Forest Science |
| 要旨本文 |
The causes of witches’ broom prevalent in Korea are diverse, such as phytoplasma, fungi, and herbicides, and it is difficult to diagnose in the field because pure culture without host cells is impossible. We developed a universal LAMP (Loop Mediated Isothermal Amplification) primer set (PRTQ kit, Speegenebio) by analyzing based on 16s rRNA regions for diagnosis of phytoplasmas diseases. When using PRTQ kit, All the phytoplasmas described above were detected within 14 minutes in the portable nucleic acid amplifier (HARU-2000, SM electrical). Phytoplasma DNA could be extracted by KN5 kit (Speegenebio) between 1 minute to 10 minutes. KN5 kit enhanced the availability of PRTQ kit by allowing rapid DNA extraction from phytoplasma-infected 6 hosts species. Therefore, diagnosis system using HARU-2000 can solve the difficulties of phytoplasma diagnosis in field. |
| 著者氏名 |
Sun Keun Lee, Yoon Hee Bae, Geon Woo Lee, Sang Sub Han, Hyeong Woo Lee |
| 著者所属 |
Warm Temperature and Subtropical Forest Research Center, National Institute of Forest Science, Jeju, Republic of Korea |
| Key word |
Phytoplasma, witches’ broom, diagnosis, LAMP, primer set |
